Journal: Methods in enzymology

Article Title: Single molecule FRET methodology for investigating glutamate receptors

doi: 10.1016/bs.mie.2021.02.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals Dulbecco’s Modified Eagle’s Medium (DMEM) GenDepot, Barker, TX Cat# CM001-050 Fetal Bovine Serum (FBS) GenDepot Cat# F0900-050 Penicillin-Streptomycin (P/S) Sigma-Aldrich, St. Louis, MO Cat# P0781 jetPRIME reagent Polyplus, Berkeley, CA Cat# 55-133 Alexa 555-C2- maleimide Invitrogen, Carlsbad, CA Cat# A20346 Alexa 647-C2-maleimide Invitrogen, Carlsbad, CA Cat# A20347 Lauryl Maltose Neopentyl Glycol Anatrace, Maumee, OH Cat# NG310 n-Dodecyl-β-D-maltoside Chem-Impex Int’l Inc., Wood Dale, IL Cat# 21950 Cholesteryl Hydrogen Succinate MP Biomedicals, Solon, OH Cat# 101379 Pierce Protease Inhibitor Mini Tablets ThermoFisher Scientific, Waltham, MA Cat# A32953 Liquinox phosphate-free detergent Fisher Scientific Cat# NC9906065 Vectabond Vector Laboratories, Burlingame, CA Cat# SP-1800 Biotin-terminated Polyethylene glycol (PEG) NOF Corp., Tokyo, Japan Cat# BI-050TS mPEG succinimidyl carbonate Laysan Bio Inc., Arab, AL Cat# MPEG-SC-5000 Sodium bicarbonate Sigma-Aldrich Cat# S6014 MS(PEG)4 Methyl-PEG-NHS-Ester Reagent Thermo Scientific Cat # 22341 Streptavidin Invitrogen Cat# 434301 Glucose Sigma-Aldrich Cat# G8270 Pyranose oxidase Sigma-Aldrich Cat# P4234 Catalase Sigma-Aldrich Cat# C1345 Ascorbic acid Sigma-Aldrich Cat# A7506 Methyl viologen Fisher Scientific Cat# AC227320010 Experimental Models: Cell Lines HEK 293T cells ATCC, Manassas VA Cat# 3216 Recombinant DNA pcDNA3.1 Invitrogen Cat# V790-20 Software and Algorithms Origin Software Origin Lab Matlab MathWorks Open in a separate window KEY RESOURCES TABLE.

Techniques: Modification, Protease Inhibitor, Plasmid Preparation, Recombinant, Software